Journal: Journal of Cancer Therapy

Article Title: Protein Expression of STAT3, pSTAT3, MMP-7 and VEGF in Colorectal Adenocarcinoma: An Immunohistochemical Study

doi: 10.4236/jct.2014.513119

Figure Lengend Snippet: Figure 1. Immunohistochemistry of poorly (a), moderately (b), (c) and well (d) differen- tiated colorectal adenocarcinoma. Immunostaining of (a) STAT3 was noted predomi- nantly in the cytoplasm of the epithelial cells (arrow); (b) pSTAT3 was demonstrated as co-existence of intense nuclear (black arrow) and very light cytoplasmic (pink arrow) staining of the epithelial cells; (c) MMP-7 was observed as a prominent cytoplasmic stain- ing in the neoplastic colonic-epithelium (arrow); and (d) VEGF was shown as predomi- nant cytoplasmic staining in the glandular tissue (arrow). Original magnification, ×400. Co-expression between STAT3, pSTAT3, MMP-7 and VEGF genes was also analysed (Table 4). Significant relationship was noted between overexpression of pSTAT3 and STAT3 (P < 0.001), pSTAT3 and VEGF (P = 0.044), pSTAT3 and MMP-7 (P = 0.003), and STAT3 and VEGF (P = 0.037) but marginal association was de- tected between STAT3 and MMP-7 (P = 0.057), and MMP-7 and VEGF (P = 0.052).

Article Snippet: These sections were immunostained with primary antibodies directed against STAT3 (rabbit anti-human, IgG H-190; polyclonal, Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), pSTAT3 (rabbit anti-human IgG Tyr705; polyclonal, Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), MMP-7 (rabbit anti-human, IgG/PUMP-1; polyclonal, Chemicon International, Temecula, CA, USA) and VEGF (mouse anti-human, IgG2a C-1; monoclonal, Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), using standard immunoperoxidase method.

Techniques: Immunohistochemistry, Immunostaining, Staining, Expressing, Over Expression